| Titre : | Chemical profiling and biological activities of some medicinal plants:aristolochia longa,bryonia dioica,ecballium elaterium and ephedra alata. |
| Auteurs : | Zouaneb Soulef, Auteur ; Benarba Bachir, Directeur de thèse |
| Type de document : | texte imprimé |
| Année de publication : | 2018 |
| Format : | 158p / 27cm |
| Note générale : | Applied biochemistry |
| Langues: | Français |
| Mots-clés: | Aristolochia longa, Bryonia dioica, echallium elaterium, ephedra alata,phytochemical,antioxidant, anti-inflammatory, anti-microbial, hemolytic. ; Sciences de la vie |
| Résumé : |
The present study was carried out to investigate the phytochemical profile and evaluate the antioxidant, anti-inflammatory, anti-microbial and hemolytic activity of an aqueous, methanolic and ethanolic extracts of four plants aristolochia longa, bryonia dioica, ecballium elaterium and ephedra alata. aqueous, methanolic and ethanolic macerations were phytochemicaly investigated using standard methods. antioxidant activity was determined using tac, dpph and frap assays. the anti-inflammatory effect was determined using egg albumin membrane denaturation and hrbc membrane stabilizing assays. the antimicrobial susceptibility tests were determined using the standard disc diffusion method. results showed that flavonoids, catechic tannins, steroids, terpenoids and alkaloids were present in the four plants. additionally, all the extracts exhibited biological activities; some of the extracts were more effective than others. the methanolic extract of bryonia dioica exhibited the highest total antioxidant activity (concentration=19.42 mg/ml aae), the best dpph free radical scavenging activity (ic5o = 0.47mg/ml) and the best reducing power (ec50 = 6.1 mg/ml). regarding anti-inflammatory activity, the aqueous extract of b. dioica exhibited the highest percentage of inhibition of protein denaturation since its ic50 (0.501 mg/ml). regarding ephedra alata, the ethanolic and aqueous extract exhibited the best tac (14.05 mg/ml aae), dpph (ic50 = 13.51 mg/ml) and frap (ec5o = 16.20 mg/mL) activities, respectively. on the other hand, the methanolic extract exhibited the highest percentage of inhibition of protein denaturation (ic5o =0.47mg/ml) and of inhibition of hemolysis (icso=1.16mg/ml). along with the methanolic extract which exhibited the highest percentage of hemolysis (ic50= 7.55 mg/ml). for the antimicrobial activity: the aqueous, methanolic and ethanolic extract exhibited the strongest antibacterial activity against escherichia coli (10.5 mm), staphylococcus aureus (13 mm) and against streptococcus sp (12 mm). moreover, our findings revealed that all the extracts of aristolochia longa exhibited a dose-dependent inhibitory effect of proteins denaturation. the methanolic extract resulted in the maximum inhibitory effect at 1000g/ml (80.53 $ 7.29%), and was significantly more effective than diclofenac (70.94 + 8.78%). similarly, all the aristolochia longa extracts exhibited a membrane stabilizir activity. at the maximum concentration (1000 g/ml), the aqueous extract resulted in the highest stabilizing I are considered, the membrane stabilizing activity could be ranked as effect (37.18+ 0.22%). When ic50 are considered, the membrane stabilizing activity e methanolic > aqueous > ethanolic. the methanolic extract of Ecballium elaterium exh methanolic extract of Ecballium elaterium exhibited the and the best dpph free radical scavenging activity while the ethanolic highest total antioxidant activity and the best dpph free radical scavenging activty while the ethanolic extract possessed the best reducing power. regarding anti-inflammatory activity, the aqueous and the extract possessed the best reducing power. regarding activity,the aqueous and the methanolic extracts exhibited the highest percentage of inhibition of protein denaturation with an ic5o of 0.11mg/ml and the highest percentage of hemolysis inhibition with an ic5o of 1.27mg/ml. taken together, our results suggest that the studied plants could be a major source of antioxidants and anti-inflammatory compounds. |
Exemplaires (2)
| Code-barres | Cote | Support | Localisation | Section | Disponibilité |
|---|---|---|---|---|---|
| t/4661 | ba31 | Livre | Bibliothèque SNV | Magasin thèses | Libre accès Disponible |
| t/4660 | ba31 | Livre | Bibliothèque SNV | Magasin thèses | Libre accès Disponible |
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